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An Efficient Biocatalytic System for Biosensing by Combining Metal-Organic Framework (MOF)-based Nanozymes and G-quadruplex (G4)-DNAzymes

Abstract : A high catalytic efficiency associated to a robust chemical structure are among the ultimate goals when developing new biocatalytic systems for biosensing applications. To get ever closer to these goals, we report here on a combination of metal-organic framework (MOF)-based nanozymes and G-quadruplex (G4)-based catalytic system known as G4-DNAzyme. This approach aims at combining the advantages of both partners (chiefly, the robustness of the former, the modularity of the latter). To this end, we used MIL-53(Fe) MOF and linked it covalently to a G4-forming sequence (F3TC), itself covalently linked to its cofactor hemin. The resulting complex (referred to as MIL-53(Fe)/G4-hemin) exhibited exquisite peroxidase-mimicking oxidation activity and an excellent robustness (being stored in water for weeks). These properties were exploited to devise a new biosensing system, based on a cascade of reactions catalyzed by the nanozyme (ABTS oxidation) and an enzyme, the alkaline phosphatase (or ALP, ascorbic acid 2-phosphate dephosphorylation). The product of the latter poisoning the former, we thus designed a biosensor for ALP (a marker of bone diseases and cancers), with a very low limit of detection (LOD, 0.02 U L-1) which is operative in human plasma samples.
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https://hal.archives-ouvertes.fr/hal-03670864
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Submitted on : Tuesday, May 17, 2022 - 6:22:08 PM
Last modification on : Wednesday, June 22, 2022 - 8:29:01 AM

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Analytical Chemistry 2022 - pe...
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Xuanxiang Mao, Fangni He, Dehui Qiu, Shijiong Wei, Rengan Luo, et al.. An Efficient Biocatalytic System for Biosensing by Combining Metal-Organic Framework (MOF)-based Nanozymes and G-quadruplex (G4)-DNAzymes. Analytical Chemistry, American Chemical Society, In press, ⟨10.1021/acs.analchem.2c00600⟩. ⟨hal-03670864⟩

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